Phi29 DNA Polymerase

Product Description: Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29, with strong strand displacement activity and high processivity (up to more than 70 kbp). The enzyme has inherent 3'-5' exonuclease activity and higher fidelity than most Taq enzymes. It is used for isothermal amplification in vitro. Specifications: Synonyms: Phi29 enzyme, Phi29 protein, Phi29 polymerase, phi29 dna polymerase, phi29 polymerase, phi29 enzyme. Applications: Multiple strand displacement amplification (MDA), Whole genome amplification (WGA), Rolling circle amplification. Concentration: 10U/µL. Unit Definition: One unit is defined as the amount of enzyme required to incorporate 0.5 pmol of dNTPs into acid-insoluble substances within 10 minutes in a total reaction volume of 50µL at 30°C. Source: E. coli strain carrying the phi29 DNA polymerase gene cloned from Bacillus subtilis phage phi29.