Phenylalanine--tRNA ligase alpha subunit (FARSA), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-508aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: ADGQVAELLL RRLEASDGGL DSAELAAELG MEHQAVVGAV KSLQALGEVI EAELRSTKHW ELTAEGEEIA REGSHEARVF RSIPPEGLAQ SELMRLPSGK VGFSKAMSNK WIRVDKSAAD GPRVFRVVDS MEDEVQRRLQ LVRGGQAEKL GEKERSELRK RKLLAEVTLK TYWVSKGSAF STSISKQETE LSPEMISSGS WRDRPFKPYN FLAHGVLPDS GHLHPLLKVR SQFRQIFLEM GFTEMPTDNF IESSFWNFDA LFQPQQHPAR DQHDTFFLRD PAEALQLPMD YVQRVKRTHS QGGYGSQGYK YNWKLDEARK NLLRTHTTSA SARALYRLAQ KKPFTPVKYF SIDRVFRNET LDATHLAEFH QIEGVVADHG LTLGHLMGVL REFFTKLGIT QLRFKPAYNP YTEPSMEVFS YHQGLKKWVE VGNSGVFRPE MLLPMGLPEN VSVIAWGLSL ERPTMIKYGI NNIRELVGHK VNLQMVYDSP LCRLDAEPRP PPTQEAA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: Expression of a gene encoding a tRNA synthetase-like protein is enhanced in tumorigenic human myeloid leukemia cells and is cell cycle stage- and differentiation-dependent.Sen S., Zhou H., Ripmaster T., Hittelman W.N., Schimmel P., White R.A.Proc. Natl. Acad. Sci. U.S.A. 94:6164-6169(1997). Function: nan