Pertussis Toxin (islet-activating protein)

Pertussis toxin (islet-activating protein) is a toxin, first isolated from B. pertussis, that is used to study G protein-coupled receptor signaling in cells and experimental autoimmune encephalomyelitis (EAE) in animals. Pertussis toxin catalyzes the transfer of the ADP-ribose moiety of NAD to the alpha subunits of heterotrimeric Gi/o proteins, resulting in the receptors being uncoupled from Gi/o proteins. Pertussis toxin is also used as an adjuvant, given with specific antigens, to immunize animals and induce EAE, an animal model of multiple sclerosis. Pertussis toxin was first described as an islet-activating protein because it caused a sustained potentiation of the secretory response of pancreatic islet cells to various stimuli that stimulate Gi-linked alpha-adrenergic receptors.CAS Number: 70323-44-3. Purity: >90% estimated by SDS-PAGE. Formulation: (Request formulation change), Each vial, when reconstituted to 500 µl with water, contains 50 µg of pertussis toxin in in 0.01 M sodium phosphate, 0.05 M sodium chloride, pH 7.0. Solubility: Water: soluble.