Periplasmic oligopeptide-binding protein (oppA), Escherichia coli, recombinant

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 27-543aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ADVPAGVTLA EKQTLVRNNG SEVQSLDPHK IEGVPESNIS RDLFEGLLVS DLDGHPAPGV AESWDNKDAK VWTFHLRKDA KWSDGTPVTA QDFVYSWQRS VDPNTASPYA SYLQYGHIAG IDEILEGKKP ITDLGVKAID DHTLEVTLSE PVPYFYKLLV HPSTSPVPKA AIEKFGEKWT QPGNIVTNGA YTLKDWVVNE RIVLERSPTY WNNAKTVINQ VTYLPIASEV TDVNRYRSGE IDMTNNSMPI ELFQKLKKEI PDEVHVDPYL CTYYYEINNQ KPPFNDVRVR TALKLGMDRD IIVNKVKAQG NMPAYGYTPP YTDGAKLTQP EWFGWSQEKR NEEAKKLLAE AGYTADKPLT INLLYNTSDL HKKLAIAASS LWKKNIGVNV KLVNQEWKTF LDTRHQGTFD VARAGWCADY NEPTSFLNTM LSNSSMNTAH YKSPAFDSIM AETLKVTDEA QRTALYTKAE QQLDKDSAIV PVYYYVNARL VKPWVGGYTG KDPLDNTYTR NMYIVKH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity. Reference: "Escherichia coli peptide binding protein OppA has a preference for positively charged peptides." Klepsch M.M., Kovermann M., Low C., Balbach J., Permentier H.P., Fusetti F., de Gier J.W., Slotboom D.J., Berntsson R.P. J. Mol. Biol. 414:75-85(2011). Function: nan