Periplasmic murein peptide-binding protein (mppA), Escherichia coli, recombinant

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 23-537aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: AEVPSGTVLA EKQELVRHIK DEPASLDPAK AVGLPEIQVI RDLFEGLVNQ NEKGEIVPGV ATQWKSNDNR IWTFTLRDNA KWADGTPVTA QDFVYSWQRL VDPKTLSPFA WFAALAGINN AQAIIDGKAT PDQLGVTAVD AHTLKIQLDK PLPWFVNLTA NFAFFPVQKA NVESGKEWTK PGNLIGNGAY VLKERVVNEK LVVVPNTHYW DNAKTVLQKV TFLPINQESA ATKRYLAGDI DITESFPKNM YQKLLKDIPG QVYTPPQLGT YYYAFNTQKG PTADQRVRLA LSMTIDRRLM TEKVLGTGEK PAWHFTPDVT AGFTPEPSPF EQMSQEELNA QAKTLLSAAG YGPQKPLKLT LLYNTSENHQ KIAIAVASMW KKNLGVDVKL QNQEWKTYID SRNTGNFDVI RASWVGDYNE PSTFLTLLTS THSGNISRFN NPAYDKVLAQ ASTENTVKAR NADYNAAEKI LMEQAPIAPI YQYTNGRLIK PWLKGYPINN PEDVAYSRTM YIVKH. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Essential for the uptake of the murein peptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate. Also transports some alpha-linked peptides such as Pro-Phe-Lys with low affinity. The transport is effected by the oligopeptide permease system. Reference: "MppA, a periplasmic binding protein essential for import of the bacterial cell wall peptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate." Park J.T., Raychaudhuri D., Li H., Normark S., Mengin-Lecreulx D. J. Bacteriol. 180:1215-1223(1998). Function: Essential for the uptake of the murein peptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate. Also transports some alpha-linked peptides such as Pro-Phe-Lys with low affinity. The transport is effected by the oligopeptide permease system.