Perfringolysin O (pfo), Clostridium perfringens, recombinant

Organism: Clostridium perfringens (strain 13 / Type A). Source: E.coli. Expression Region: 29-500aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: KDITDKNQSI DSGISSLSYN RNEVLASNGD KIESFVPKEG KKTGNKFIVV ERQKRSLTTS PVDISIIDSV NDRTYPGALQ LADKAFVENR PTILMVKRKP ININIDLPGL KGENSIKVDD PTYGKVSGAI DELVSKWNEK YSSTHTLPAR TQYSESMVYS KSQISSALNV NAKVLENSLG VDFNAVANNE KKVMILAYKQ IFYTVSADLP KNPSDLFDDS VTFNDLKQKG VSNEAPPLMV SNVAYGRTIY VKLETTSSSK DVQAAFKALI KNTDIKNSQQ YKDIYENSSF TAVVLGGDAQ EHNKVVTKDF DEIRKVIKDN ATFSTKNPAY PISYTSVFLK DNSVAAVHNK TDYIETTSTE YSKGKINLDH SGAYVAQFEV AWDEVSYDKE GNEVLTHKTW DGNYQDKTAH YSTVIPLEAN ARNIRIKARE CTGLAWEWWR DVISEYDVPL TNNINVSIWG TTLYPGSSIT YN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation. Reference: "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002). Function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.