Peptidylglycine alpha-hydroxylating monooxygenase (Phm), Drosophila melanogaster, recombinant

Organism: Drosophila melanogaster (Fruit fly). Source: E.coli. Expression Region: 25-365aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: LVKEGDYQNS LYQQNLESNS ATGATASFPF LMPNVSPQTP DLYLCTPIKV DPTTTYYIVG FNPNATMNTA HHMLLYGCGE PGTSKTTWNC GEMNRASQEE SASPCGPHSN SQIVYAWARD AQKLNLPEGV GFKVGKNSPI KYLVLQVHYA HIDKFKDGST DDSGVFLDYT EEPRKKLAGT LLLGTDGQIP AMKTEHLETA CEVNEQKVLH PFAYRVHTHG LGKVVSGYRV RTNSDGEQEW LQLGKRDPLT PQMFYNTSNT DPIIEGDKIA VRCTMQSTRH RTTKIGPTNE DEMCNFYLMY YVDHGETLNM KFCFSQGAPY YFWSNPDSGL HNIPHIEAST L. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Monooxygenase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Produces an unstable peptidyl(2-hydroxyglycine) intermediate. C-terminal amidation of peptides is required for normal developmental transitions and for biosynthesis of secretory peptides throughout the life. Reference: n/a. Function: nan