Peptidase 1 (DERP1), Dermatophagoides pteronyssinus, recombinant

Organism: Dermatophagoides pteronyssinus (European house dust mite). Source: E.coli. Expression Region: 99-320aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: TNACSINGNA PAEIDLRQMR TVTPIRMQGG CGSCWAFSGV AATESAYLAY RNQSLDLAEQ ELVDCASQHG CHGDTIPRGI EYIQHNGVVQ ESYYRYVARE QSCRRPNAQR FGISNYCQIY PPNVNKIREA LAQTHSAIAV IIGIKDLDAF RHYDGRTIIQ RDNGYQPNYH AVNIVGYSNA QGVDYWIVRN SWDTNWGDNG YGYFAANIDL MMIEEYPYVV IL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues. Reference: "Cloning and expression of DNA coding for the major house dust mite allergen Der p 1 in Escherichia coli."Thomas W.R., Stewart G.A., Simpson R.J., Chua K.Y., Plozza T.M., Dilworth R.J., Nisbet A., Turner K.J.Int. Arch. Allergy Appl. Immunol. 85:127-129(1988). Function: Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues.