Pectin lyase 1A, Bacillus subtilis

Pectin lyase 1A (BsPly1A), assigned the E.C. number 4.2.2.10, is a derivative of Bacillus subtilis. It is an enzyme that acts on 1,4-alpha-D-galacturonan methyl ester. The recombinant BsPly1A, purified from Escherichia coli, is a single-domain Pectate Lyase family 1 (PL1) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Pectin lyases. Family: PL1. Ec Number: 4.2.2.10. Organism: Bacillus subtilis. Architecture: PL1. Optimal pH: 8. Optimal Temperature: 60°C. Substrates: pectins