Pectate lyase 5, Ambrosia artemisiifolia, recombinant

Organism: Ambrosia artemisiifolia (Short ragweed). Source: Yeast. Expression Region: 26-396aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AEDLQEILPV NETRRLTTSG AYNIIDGCWR GKADWAENRK ALADCAQGFG KGTVGGKDGD IYTVTSELDD DVANPKEGTL RFGAAQNRPL WIIFERDMVI RLDKEMVVNS DKTIDGRGAK VEIINAGFTL NGVKNVIIHN INMHDVKVNP GGLIKSNDGP AAPRAGSDGD AISISGSSQI WIDHCSLSKS VDGLVDAKLG TTRLTVSNSL FTQHQFVLLF GAGDENIEDR GMLATVAFNT FTDNVDQRMP RCRHGFFQVV NNNYDKWGSY AIGGSASPTI LSQGNRFCAP DERSKKNVLG RHGEAAAESM KWNWRTNKDV LENGAIFVAS GVDPVLTPEQ SAGMIPAEPG ESALSLTSSA GVLSCQPGAP C. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has pectate lyase activity. Reference: "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)." Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K. Int. Arch. Allergy Appl. Immunol. 96:296-304(1991). Function: Has pectate lyase activity.