PDGF-B (human, recombinant)

PDGF-B is a secreted growth factor and member of the PDGF family. It is composed of a signal peptide, a pro-peptide sequence, a growth factor domain containing a cystine knot, and a C-terminal tail. PDGF-B is expressed at high levels in the heart, placenta, and fetal kidney and in the brain in the substantia nigra. It exists as either a homodimer, PDGF-BB, which can activate PDGFRalpha or PDGFRbeta, or a heterodimer with PDGF-A, PDGF-AB, which can only activate PDGFRalpha, and induces signaling through multiple pathways, including ERK/MAPK, PI3K/Akt/mTOR, PLC/PKC, and JAK/STAT. PDGF-B is a potent mitogen for cells of mesenchymal origin and is necessary for vascular development and vascular smooth muscle cell proliferation in the CNS, lungs, heart, placenta, and skin. A fusion between the genes encoding PDGF-B and collagen 1A1, PDGFB and COL1A1, leads to overproduction of PDGF-B and is associated with various benign and malignant tumors, including dermatofibrosarcoma protuberans. Formulations containing PDGF-B have been used in the treatment of diabetic neuropathic foot ulcers. Cayman's PDGF-B (human, recombinant) protein can be used for binding assays. This protein consists of 124 amino acids and has a calculated molecular weight of 14. kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 20 kDa due to glycosylation.