P2Y purinoceptor 13 (P2yr13), mouse, recombinant

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 1-377aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MLGTINTTGM QGFNKSERCP RDTRMTQLLF PVLYTVVFLA GILLNTVALW VFVHIPSNST FIVYLKNTLV ADLIMALMLP FKILSDSHLA PWQLRGFVCT LSSVVFYETM YVGIMMLGLI AFDRFLKIIM PFRKTFVKKT AFAKTVSISV WSLMFFISLP NMILNKEATP SSVKKCASLK SPLGLWWHQV VSHTCQFIFW AVFILMLLFY AVITKKVYNS YRKFRSKDSR HKRLEVKVFI VMAVFFVCFA PLHFVRIPYT YSQTTNKTDC RLENQLFIAK EATLFLATTN ICMDPLIYII LCKKFTQKVP CVRWGKARTA GSSEDHHSSQ TDNITLA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system. Reference: "Chronic pharmacological activation of P2Y13 receptor in mice decreases HDL-cholesterol level by increasing hepatic HDL uptake and bile acid secretion." Serhan N., Cabou C., Verdier C., Lichtenstein L., Malet N., Perret B., Laffargue M., Martinez L.O. Biochim Biophys Acta 1831:719-725(2013). Function: nan