Outer membrane protein A (ompA), recombinant Escherichia coli

Organism: Escherichia coli (strain K12). Source: in vitro E.coli expression system. Expression Region: 22-346aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: APKDNTWYTG AKLGWSQYHD TGFINNNGPT HENQLGAGAF GGYQVNPYVG FEMGYDWLGR MPYKGSVENG AYKAQGVQLT AKLGYPITDD LDIYTRLGGM VWRADTKSNV YGKNHDTGVS PVFAGGVEYA ITPEIATRLE YQWTNNIGDA HTIGTRPDNG MLSLGVSYRF GQGEAAPVVA PAPAPAPEVQ TKHFTLKSDV LFNFNKATLK PEGQAALDQL YSQLSNLDPK DGSVVVLGYT DRIGSDAYNQ GLSERRAQSV VDYLISKGIP ADKISARGMG ESNPVTGNTC DNVKQRAALI DCLAPDRRVE IEVKGIKDVV TQPQA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes. Reference: "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006). Function: Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes.