Outer membrane porin F (oprF), Pseudomonas aeruginosa, recombinant

Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). Source: E.coli. Expression Region: 25-350aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. Target Protein Sequence: QGQNSVEIEA FGKRYFTDSV RNMKNADLYG GSIGYFLTDD VELALSYGEY HDVRGTYETG NKKVHGNLTS LDAIYHFGTP GVGLRPYVSA GLAHQNITNI NSDSQGRQQM TMANIGAGLK YYFTENFFAK ASLDGQYGLE KRDNGHQGEW MAGLGVGFNF GGSKAAPAPE PVADVCSDSD NDGVCDNVDK CPDTPANVTV DANGCPAVAE VVRVQLDVKF DFDKSKVKEN SYADIKNLAD FMKQYPSTST TVEGHTDSVG TDAYNQKLSE RRANAVRDVL VNEYGVEGGR VNAVGYGESR PVADNATAEG RAINRRVEAE VEAEAK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium. Reference: n/a. Function: nan