NZYTaq II DNA Polymerase with 5x Gel Load

NZYTaq II DNA polymerase belongs to a new generation of Taq-derived DNA polymerases that was engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension) under minimal optimization conditions. NZYTaq II DNA polymerase supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. NZYTaq II is provided with 5x Gel Load Reaction Buffer allowing reactions to be loaded directly into gels without the extra adding of loading dye. This Gel Load Reaction Buffer is composed by a blue and yellow dye. The blue dye migrates at the same rate as a 3-5 kb DNA fragment in a 1% (w/v) agarose gel. The yellow dye migrates at a rate faster than primers (<50 bp) in a 1% (v/v) agarose gel. The 5x Gel Load Reaction Buffer is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. NZYTaq II DNA polymerase lacks 3'->5' exonuclease activity. Resulting PCR products have an A overhang and are suitable for cloning with NZYTech's TA PCR cloning kits (MB053 or MB137). Components: * NZYTaq II DNA polymerase (5 U/µL) * Gel Load Reaction buffer (5x) * MgCl2 Solution (50 mM). Shipping Conditions: Room Temperature. Storage Conditions: -85°C to -15°C. Application: Routine PCR, TA Cloning. Features: Standard. Product Length: 0-6 kb. Format: Enzyme/Protein. Product Category: End-Point PCR. Concentration: 5 U/µL. Hot Start: No