NZY Turbonuclease

NZY Turbonuclease is a highly efficient, recombinant endonuclease derived from the bacterium Serratia marcescens. This enzyme is engineered to exhibit robust nuclease activity, making it an ideal choice for a variety of molecular biology applications where the removal of nucleic acids is required. The enzyme undergoes rigorous purification to eliminate host proteins and other potential contaminants, thus ensuring dependable and consistent performance across a spectrum of applications. NZY Turbonuclease shows remarkable enzymatic activity over a broad temperature range (20°C to 50°C) and pH spectrum (pH 7 to 10), facilitating its integration into various workflows and protocols. Additionally, it maintains effectiveness in the presence of salt concentrations up to 0.2 M but requires the presence of Mg2+ (optimum 2 mM). NZY Turbonuclease activity is equivalent to, and in some cases slightly higher than, Benzonase. This enzyme is invaluable in applications such as clearing cell lysates, removing nucleic acid contaminants from recombinant protein preparations, and enhancing the clarity of microbial culture broths. NZY Turbonuclease is compatible with most used buffers and additives in molecular biology, including reducing agents, demonstrating minimal activity loss. The enzyme is capable of digesting both native and heat-denatured DNA and RNA molecules independently of being single- or double-stranded, circular, linear or supercoiled and is available at a high concentration of 500 U/µL (500 kU/mL), offering up to 50 times more activity and 350% greater catalytic efficiency compared to DNase I. The enzyme is RNase-free and provided in a recombinant format, ensuring that it meets the stringent requirements of various scientific and clinical research protocols. Components: NZY Turbonuclease/10x Reaction Buffer for NZY Turbonuclease