Nucleoprotein (N) (Q384H) (Active), recombinant Severe acute respiratory syndrome coronavirus 2

Organism: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2). Source: E.coli. Expression Region: 1-419aa(Q384H). Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTGAIKLD DKDPNFKDQV ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPHRQKKQQ TVTLLPAADL DDFSKQLQQS MSSADSTQA. Purity: Greater than 84% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: 1Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M11)) at 2 µg/ml can bind N Mouse Monoclonal Antibody(CSB-MA33255A2m), the EC50 of N Mouse Monoclonal Antibody is 7.617-10.60 ng/ml. 2Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M11)) at 2 µg/ml can bind N Recombinant Antibody(CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody is 2.785-4.100 ng/ml. Form: Lyophilized powder. Buffer: Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: n/a. Reference: n/a. Function: nan