Nucleoprotein (N), Lassa virus, recombinant

Organism: Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) (LASV). Source: E.coli. Expression Region: 1-569aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MSASKEIKSF LWTQSLRREL SGYCSNIKLQ VVKDAQALLH GLDFSEVSNV QRLMRKERRD DNDLKRLRDL NQAVNNLVEL KSTQQKSILR VGTLTSDDLL ILAADLEKLK SKVIRTERPL SAGVYMGNLS SQQLDQRRAL LNMIGMSGGN QGARAGRDGV VRVWDVKNAE LLNNQFGTMP SLTLACLTKQ GQVDLNDAVQ ALTDLGLIYT AKYPNTSDLD RLTQSHPILN MIDTKKSSLN ISGYNFSLGA AVKAGACMLD GGNMLETIKV SPQTMDGILK SILKVKKALG MFISDTPGER NPYENILYKI CLSGDGWPYI ASRTSITGRA WENTVVDLES DGKPQKADSN NSSKSLQSAG FTAGLTYSQL MTLKDAMLQL DPNAKTWMDI EGRPEDPVEI ALYQPSSGCY IHFFREPTDL KQFKQDAKYS HGIDVTDLFA TQPGLTSAVI DALPRNMVIT CQGSDDIRKL LESQGRKDIK LIDIALSKTD SRKYENAVWD QYKDLCHMHT GVVVEKKKRG GKEEITPHCA LMDCIMFDAA VSGGLNTSVL RAVLPRDMVF RTSTPRVVL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (By similarity). Reference: "Nucleotide sequence of the Lassa virus (Josiah strain) S genome RNA and amino acid sequence comparison of the N and GPC proteins to other arenaviruses."Auperin D.D., McCormick J.B.Virology 168:421-425(1989). Function: Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (By similarity).