Nucleoprotein (N), Bat coronavirus HKU9, recombinant

Organism: Bat coronavirus HKU9 (BtCoV) (BtCoV/HKU9). Source: Baculovirus. Expression Region: 1-468aa. Protein Length: Full Length. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: MSGRNRSRSG TPSPKVTFKQ ESDGSDSESE RRNGNRNGAR PKNNNSRGSA PKPEKPKAAP PQNVSWFAPL VQTGKAELRF PRGEGVPVSQ GVDSTYEHGY WLRTQRSFQK GGKQVLANPR WYFYYTGTGR FGDLRFGTKN PDIVWVGQEG ANINRLGDMG TRNPSNDGAI PVQLAGGIPK GFYAEGRGSR GNSRSSSRNS SRASSRGNSR ASSRGASPGR PAANPSTEPW MAYLVQKLER LESQVSGTKP ATKNPVQVTK NEAAANAKKL RHKRTAHKGS GVTVNYGRRG PGDLEGNFGD REMIKLGTDD PRFAAAAQMA PNVSSFLFMS HLSTRDEDDA LWLHYKGAIK LPKDDPNYEQ WTKILAENLN AYKDFPPTEP KKDKKKKEET AQDTVIFEDA STGTDQTVVK VWVKDQDAQT DDEWLGGDET VYEDEDDRPK TQRRHKKRGS TASRVTIADP TNAGAERS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Reference: "Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features." Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y. J. Virol. 81:1574-1585(2007). Function: nan