Nuclear transcription factor Y subunit A-3 (NFYA3), Arabidopsis thaliana, recombinant

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: E.coli. Expression Region: 1-340aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MMHQMLNKKD SATHSTLPYL NTSISWGVVP TDSVANRRGS AESLSLKVDS RPGHIQTTKQ ISFQDQDSSS TQSTGQSYTE VASSGDDNPS RQISFSAKSG SEITQRKGFA SNPKQGSMTG FPNIHFAPAQ ANFSFHYADP HYGGLLAATY LPQAPTCNPQ MVSMIPGRVP LPAELTETDP VFVNAKQYHA IMRRRQQRAK LEAQNKLIRA RKPYLHESRH VHALKRPRGS GGRFLNTKKL LQESEQAAAR EQEQDKLGQQ VNRKTNMSRF EAHMLQNNKD RSSTTSGSDI TSVSDGADIF GHTEFQFSGF PTPINRAMLV HGQSNDMHGG GDMHHFSVHI. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters. Reference: Multiple genes encoding the conserved CCAAT-box transcription factor complex are expressed in Arabidopsis.Edwards D., Murray J.A.H., Smith A.G.Plant Physiol. 117:1015-1022(1998). Function: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters.