NHE4, Rat (Na, H Exchanger 4) (Control Peptide)

Control peptide for N2018-07 (antiserum) and N2018-08 (affinity purified antibody). Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. , NHE4 (rat, 717aa, rat chromosome 9 and human chromosome 2) expression is most abundant in rat stomach followed by colon and intestine, and in low levels in rat kidney, uterus, and brain , (1). Unlike other NHEs, NHE4 has no amiloride-sensitive Na+ uptake under basal or acid-loaded isomotic conditions. Applications: Suitable for use in ELISA and antibody blocking experiments. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide/well. Optimal dilutions to be determined by the researcher. , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.