NF-kappa-B-repressing factor (NKRF), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 4-688aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ILQMAEGIDI GEMPSYDLVL SKPSKGQKRH LSTCDGQNPP KKQAGSKFHA RPRFEPVHFV ASSSKDERQE DPYGPQTKEV NEQTHFASMP RDIYQDYTQD SFSIQDGNSQ YCDSSGFILT KDQPVTANMY FDSGNPAPST TSQQANSQST PEPSPSQTFP ESVVAEKQYF IEKLTATIWK NLSNPEMTSG SDKINYTYML TRCIQACKTN PEYIYAPLKE IPPADIPKNK KLLTDGYACE VRCQNIYLTT GYAGSKNGSR DRATELAVKL LQKRIEVRVV RRKFKHTFGE DLVVCQIGMS SYEFPPALKP PEDLVVLGKD ASGQPIFNAS AKHWTNFVIT ENANDAIGIL NNSASFNKMS IEYKYEMMPN RTWRCRVFLQ DHCLAEGYGT KKTSKHAAAD EALKILQKTQ PTYPSVKSSQ CHTGSSPRGS GKKKDIKDLV VYENSSNPVC TLNDTAQFNR MTVEYVYERM TGLRWKCKVI LESEVIAEAV GVKKTVKYEA AGEAVKTLKK TQPTVINNLK KGAVEDVISR NEIQGRSAEE AYKQQIKEDN IGNQLLRKMG WTGGGLGKSG EGIREPISVK EQHKREGLGL DVERVNKIAK RDIEQIIRNY ARSESHTDLT FSRELTNDER KQIHQIAQKY GLKSKSHGVG HDRYLVVGRK RRKEDLLDQL KQEGQVGHYE LVMPQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Interacts with a specific negative regulatory elent (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription. Reference: Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011). Function: Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.