Neurofilament light polypeptide (NEFL), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-543aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: SSFSYEPYYS TSYKRRYVET PRVHISSVRS GYSTARSAYS SYSAPVSSSL SVRRSYSSSS GSLMPSLENL DLSQVAAISN DLKSIRTQEK AQLQDLNDRF ASFIERVHEL EQQNKVLEAE LLVLRQKHSE PSRFRALYEQ EIRDLRLAAE DATNEKQALQ GEREGLEETL RNLQARYEEE VLSREDAEGR LMEARKGADE AALARAELEK RIDSLMDEIS FLKKVHEEEI AELQAQIQYA QISVEMDVTK PDLSAALKDI RAQYEKLAAK NMQNAEEWFK SRFTVLTESA AKNTDAVRAA KDEVSESRRL LKAKTLEIEA CRGMNEALEK QLQELEDKQN ADISAMQDTI NKLENELRTT KSEMARYLKE YQDLLNVKMA LDIEIAAYRK LLEGEETRLS FTSVGSITSG YSQSSQVFGR SAYGGLQTSS YLMSTRSFPS YYTSHVQEEQ IEVEETIEAA KAEEAKDEPP SEGEAEEEEK DKEEAEEEEA AEEEEAAKEE SEEAKEEEEG GEGEEGEETK EAEEEEKKVE GAGEEQAAKK KD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. Reference: "The structure of a human neurofilament gene (NF-L): a unique exon-intron organization in the intermediate filament gene family."Julien J.-P., Grosveld F., Yazdanbaksh K., Flavell D., Meijer D., Mushynski W.Biochim. Biophys. Acta 909:10-20(1987). Function: nan