Neuroepithelial cell-transforming gene 1 protein (NET1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-542aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MVAHDETGGL LPIKRTIRVL DVNNQSFREQ EEPSNKRVRP LARVTSLANL ISPVRNGAVR RFGQTIQSFT LRGDHRSPAS AQKFSSRSTV PTPAKRRSSA LWSEMLDITM KESLTTREIR RQEAIYEMSR GEQDLIEDLK LARKAYHDPM LKLSIMSEEE LTHIFGDLDS YIPLHEDLLT RIGEATKPDG TVEQIGHILV SWLPRLNAYR GYCSNQLAAK ALLDQKKQDP RVQDFLQRCL ESPFSRKLDL WSFLDIPRSR LVKYPLLLKE ILKHTPKEHP DVQLLEDAIL IIQGVLSDIN LKKGESECQY YIDKLEYLDE KQRDPRIEAS KVLLCHGELR SKSGHKLYIF LFQDILVLTR PVTRNERHSY QVYRQPIPVQ ELVLEDLQDG DVRMGGSFRG AFSNSEKAKN IFRIRFHDPS PAQSHTLQAN DVFHKQQWFN CIRAAIAPFQ SAGSPPELQG LPELHEECEG NHPSARKLTA QRRASTVSSV TQVEVDENAY RCGSGMQMAE DSKSLKTHQT QPGIRRARDK ALSGGKRKET LV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. Reference: The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage.Srougi M.C., Burridge K.PLoS ONE 6:E17108-E17108(2011). Function: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.