NADPH Cytochrome P450 Reductase (human, recombinant)

NADPH cytochrome P450 reductase (POR) is a flavoprotein and key electron donor to cytochrome P450s (CYPs). It is composed of an N-terminal membrane anchor domain and FMN-binding domain, which is linked to a C-terminal FAD-binding domain and NADPH-binding domain and localized to the cytosolic side of the endoplasmic reticulum. POR transfers electrons from NADPH through FAD and FMN coenzymes into the iron of the prosthetic heme group of CYPs. Cortical levels of POR are increased in the APPswe/PSEN1dE9 transgenic mouse model of Alzheimer's disease. Deficiency in POR is associated with ambiguous genitalia and the disruption of steroid metabolism in patients with Antley-Bixler syndrome. Cayman's NADPH Cytochrome P450 Reductase (human, recombinant) can be used for enzyme activity assays.Synonyms: CPR, CYPOR, NADPH Hemoprotein Reductase, NCPR, P450R, POR. Purity: >90% estimated by SDS-PAGE. Source: Active recombinant human C-terminal His-tagged NADPH cytochrome P450 reductase expressed in E. coli. Amino Acids: 24-677. MW: 76.4 kDa. Formulation: (Request formulation change), 50 mM potassium phosphate, pH 7.6, with 10% glycerol. UniProt Accession #: P16435.