NADPH:adrenodoxin oxidoreductase, mitochondrial (FDXR), bovine, recombinant

Organism: Bos taurus (Bovine). Source: E.coli. Expression Region: 33-492aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: STQEQTPQIC VVGSGPAGFY TAQHLLKHHS RAHVDIYEKQ LVPFGLVRFG VAPDHPEVKN VINTFTQTAR SDRCAFYGNV EVGRDVTVQE LQDAYHAVVL SYGAEDHQAL DIPGEELPGV FSARAFVGWY NGLPENRELA PDLSCDTAVI LGQGNVALDV ARILLTPPDH LEKTDITEAA LGALRQSRVK TVWIVGRRGP LQVAFTIKEL REMIQLPGTR PMLDPADFLG LQDRIKEAAR PRKRLMELLL RTATEKPGVE EAARRASASR AWGLRFFRSP QQVLPSPDGR RAAGIRLAVT RLEGIGEATR AVPTGDVEDL PCGLVLSSIG YKSRPIDPSV PFDPKLGVVP NMEGRVVDVP GLYCSGWVKR GPTGVITTTM TDSFLTGQIL LQDLKAGHLP SGPRPGSAFI KALLDSRGVW PVSFSDWEKL DAEEVSRGQA SGKPREKLLD PQEMLRLLGH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Serves as the first electron transfer protein in all the mitochondrial P450 systems including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver. Reference: "Adrenoferredoxin-binding peptide of NADPH-adrenoferredoxin reductase." Hamamoto I., Kurokohchi K., Tanaka S., Ichikawa Y. Biochim. Biophys. Acta 953:207-213(1988). Function: Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.