NaDC-3, Human, Control Peptide (Sodium Dicarboxylate Transporter)

The sodium-dicarboxylate cotransporters (Solute carrier family 13) , which transport succinate and other Krebs cycle intermediates, fall into 2 categories based on their substrate affinity, low affinity and high affinity. Both the low- and high-affinity transporters play an important role in the handling of citrate by the kidneys. The two different Na+-coupled dicarboxylate transporters (NADC) have been identified in mammalian tissues. These are NADC1and NADC3 (NADC2 is found in C. Elegans). NADC1 is Na+-coupled, electrogenic, and exhibits low affinity for its dicarboxylatesubstrates. This isoform is expressedprimarily in the brush border membrane of intestinal and renalepithelial cells. The physiological function of NADC1 is to absorbthe intermediates of the citric acid cycle, such as citrate, succinate,-ketoglutarate, fumarate, and malate, in the intestine and kidney.NADC3 is also a Na+-coupled and electrogenic dicarboxylate transporter, but it exhibitsrelatively higher affinity for its substrates compared with NADC1. The NADC3is expressed primarily in the basolateral membrane of intestinaland renal epithelial cells. However, it is also found in tissuessuch as liver, placenta, and brain. NADC3 in the kidney is involvedin generating the driving force for the organic anion transporterOAT1 to facilitate the active entry of organic anions into thetubular cells across the basolateral membrane. In the brain,NADC3 mediates the cellular uptake of N-acetylaspartate, a processclosely linked to myelination. Therefore, the physiologicalfunctions of the NADCs may extend beyond the mediation of cellularentry of citric acid cycle intermediates. Recently, a third member of this familyin mammals has been identified, Na+-coupled citrate transporter (NACT), mediates the cellular uptakeof citrate in a Na+-coupled manner. , NADC-3, (SCL13A3 gene) a sodium dependent high- affinity dicarboxylate transporter with 600aa each in mouse and rat, it's a 602aa sequence in human ( chr 20q12-q13.1) expressed in kidney, brain, liver and placenta. NADC-3 exhibits 48% identity with NADC-1. The transport function of NADC-3 shows a sigmoidal relationship with regard to Na+ concentration, NADC3 accepts a number of dicarboxylates including dimethyl-succinate as substrates and excludes monocarboxylates. Transport of succinate is markedly influenced by pH, the transport gradually decreases when pH is acidic. Source: 14aa peptide of human NADC-3, Location: ~C-terminal, Applications: Suitable for use in ELISA as a control peptide for N0016C. Other applications not tested. Recommended Dilution: ELISA: Coat ELISA plates at 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.