NAD (+) hydrolase SARM1 (SARM1), human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 28-724aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: LAVPGPDGGG GTGPWWAAGG RGPREVSPGA GTEVQDALER ALPELQQALS ALKQAGGARA VGAGLAEVFQ LVEEAWLLPA VGREVAQGLC DAIRLDGGLD LLLRLLQAPE LETRVQAARL LEQILVAENR DRVARIGLGV ILNLAKEREP VELARSVAGI LEHMFKHSEE TCQRLVAAGG LDAVLYWCRR TDPALLRHCA LALGNCALHG GQAVQRRMVE KRAAEWLFPL AFSKEDELLR LHACLAVAVL ATNKEVEREV ERSGTLALVE PLVASLDPGR FARCLVDASD TSQGRGPDDL QRLVPLLDSN RLEAQCIGAF YLCAEAAIKS LQGKTKVFSD IGAIQSLKRL VSYSTNGTKS ALAKRALRLL GEEVPRPILP SVPSWKEAEV QTWLQQIGFS KYCESFREQQ VDGDLLLRLT EEELQTDLGM KSGITRKRFF RELTELKTFA NYSTCDRSNL ADWLGSLDPR FRQYTYGLVS CGLDRSLLHR VSEQQLLEDC GIHLGVHRAR ILTAAREMLH SPLPCTGGKP SGDTPDVFIS YRRNSGSQLA SLLKVHLQLH GFSVFIDVEK LEAGKFEDKL IQSVMGARNF VLVLSPGALD KCMQDHDCKD WVHKEIVTAL SCGKNIVPII DGFEWPEPQV LPEDMQAVLT FNGIKWSHEY QEATIEKIIR FLQGRSSRDS SAGSDTSLEG AAPMGPT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway which plays a pivotal role in activating axonal degeneration following injury. Promotes Wallerian degeneration an injury-induced axonal death pathway which involves degeneration of an axon distal to the injury site. Can activate neuronal death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response. Inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38. Reference: "SARM inhibits both TRIF- and MyD88-mediated AP-1 activation." Peng J., Yuan Q., Lin B., Panneerselvam P., Wang X., Luan X.L., Lim S.K., Leung B.P., Ho B., Ding J.L. Eur. J. Immunol. 40:1738-1747(2010). Function: nan