Multiple inositol polyphosphate phosphatase 1 (Minpp1), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 31-481aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SLPGRGDPVA SVLSPYFGTK TRYEDVNPWL LGDPVAPRRD PELLAGTCTP VQLVALIRHG TRYPTTKQIR KLRQLQGLLQ TRESVDGGSR VAAALDQWPL WYDDWMDGQL VEKGRQDMRQ LALRLAALFP DLFCRENYGR LRLITSSKHR CVDSSAAFLQ GLWQHYHPGL PPPDVSDMEC DPPRVNDKLM RFFDHCEKFL TEVERNATAL YHVEAFKTGP EMQTVLKKVA ATLQVPVNNL NADLIQVAFF TCSFDLAIQG VHSPWCDVFD VDDAKVLEYL NDLKQYWKRS YGYAINSRSS CNLFQDIFLH LDKAVEQKQR SQPVSSSVIL QFGHAETLLP LLSLMGYFKD KEPLTAYNFE EQVHREFRSG HIVPYASNLI FVLYHCEDAQ TPQEKFQIQM LLNEKVLPLA HSQKTVALYE DLKNHYQDIL QSCQTSKECN LPKVNITSDE L. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acts as a phosphoinositide 5- and phosphoinositide 6-phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3-phosphoglycerate. May play a role in bone development (endochondral ossification). Reference: The human and rat forms of multiple inositol polyphosphate phosphatase functional homology with a histidine acid phosphatase up-regulated during endochondral ossification.Caffrey J.J., Hidaka K., Matsuda M., Hirata M., Shears S.B.FEBS Lett. 442:99-104(1999). Function: Acts as a phosphoinositide 5- and phosphoinositide 6-phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3-phosphoglycerate. May play a role in bone development (endochondral ossification). May play a role in the transition of chondrocytes from proliferation to hypertrophy (By similarity).