Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3 (PLOD3), human, recombina

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 25-738aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: SDRPRGRDPV NPEKLLVITV ATAETEGYLR FLRSAEFFNY TVRTLGLGEE WRGGDVARTV GGGQKVRWLK KEMEKYADRE DMIIMFVDSY DVILAGSPTE LLKKFVQSGS RLLFSAESFC WPEWGLAEQY PEVGTGKRFL NSGGFIGFAT TIHQIVRQWK YKDDDDDQLF YTRLYLDPGL REKLSLNLDH KSRIFQNLNG ALDEVVLKFD RNRVRIRNVA YDTLPIVVHG NGPTKLQLNY LGNYVPNGWT PEGGCGFCNQ DRRTLPGGQP PPRVFLAVFV EQPTPFLPRF LQRLLLLDYP PDRVTLFLHN NEVFHEPHIA DSWPQLQDHF SAVKLVGPEE ALSPGEARDM AMDLCRQDPE CEFYFSLDAD AVLTNLQTLR ILIEENRKVI APMLSRHGKL WSNFWGALSP DEYYARSEDY VELVQRKRVG VWNVPYISQA YVIRGDTLRM ELPQRDVFSG SDTDPDMAFC KSFRDKGIFL HLSNQHEFGR LLATSRYDTE HLHPDLWQIF DNPVDWKEQY IHENYSRALE GEGIVEQPCP DVYWFPLLSE QMCDELVAEM EHYGQWSGGR HEDSRLAGGY ENVPTVDIHM KQVGYEDQWL QLLRTYVGPM TESLFPGYHT KARAVMNFVV RYRPDEQPSL RPHHDSSTFT LNVALNHKGL DYEGGGCRFL RYDCVISSPR KGWALLHPGR LTHYHEGLPT TWGTRYIMVS FVDP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. Reference: "Primary structure, tissue distribution, and chromosomal localization of a novel isoform of lysyl hydroxylase (lysyl hydroxylase 3)." Valtavaara M., Szpirer C., Szpirer J., Myllylae R. J. Biol. Chem. 273:12881-12886(1998). Function: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.