Multidrug resistance-associated protein 1 (ABCC1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1248-1531aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: VRMSSEMETN IVAVERLKEY SETEKEAPWQ IQETAPPSSW PQVGRVEFRN YCLRYREDLD FVLRHINVTI NGGEKVGIVG RTGAGKSSLT LGLFRINESA EGEIIIDGIN IAKIGLHDLR FKITIIPQDP VLFSGSLRMN LDPFSQYSDE EVWTSLELAH LKDFVSALPD KLDHECAEGG ENLSVGQRQL VCLARALLRK TKILVLDEAT AAVDLETDDL IQSTIRTQFE DCTVLTIAHR LNTIMDYTRV IVLDKGEIQE YGAPSDLLQQ RGLFYSMAKD AGLV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. Reference: "Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line." Cole S.P.C., Bhardwaj G., Gerlach J.H., Mackie J.E., Grant C.E., Almquist K.C., Stewart A.J., Kurz E.U., Duncan A.M.V., Deeley R.G. Science 258:1650-1654(1992). Function: Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.