Mucosal addressin cell adhesion molecule 1 (Madcam1), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 22-364aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: QSFQVNPPES EVAVAMGTSL QITCSMSCDE GVARVHWRGL DTSLGSVQTL PGSSILSVRG MLSDTGTPVC VGSCGSRSFQ HSVKILVYAF PDQLVVSPEF LVPGQDQVVS CTAHNIWPAD PNSLSFALLL GEQRLEGAQA LEPEQEEEIQ EAEGTPLFRM TQRWRLPSLG TPAPPALHCQ VTMQLPKLVL THRKEIPVLQ SQTSPKPPNT TSAEPYILTS SSTAEAVSTG LNITTLPSAP PYPKLSPRTL SSEGPCRPKI HQDLEAGWEL LCEASCGPGV TVRWTLAPGD LATYHKREAG AQAWLSVLPP GPMVEGWFQC RQDPGGEVTN LYVPGQVTPN SSS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. Reference: "MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1."Briskin M.J., McEvoy L.M., Butcher E.C.Nature 363:461-464(1993). Function: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.