mTOR/mLST8/Raptor Complex (human, recombinant)

mTORC1 is a protein complex composed of the serine/threonine kinase mammalian target of rapamycin (mTOR), TOR complex subunit LST8 (mLST8), regulatory-associated protein of mTOR (Raptor), proline-rich Akt substrate 40 kDa (PRAS40), and DEP domain-containing mTOR-interacting protein (Deptor) that functions as a nutrient sensor and regulates cell growth and metabolism. PRAS40 and Deptor act as negative regulators of mTORC1 activity, and mLST8 and Raptor as scaffold proteins for substrates such as 4E-BP1 and p70 ribosomal S6 kinase 1 (p70S6K1). mTORC1 localizes to the plasma membrane, lysosome, and nucleus depending on its upstream activation signal, which include AMPK, PI3K, and Akt. Activation of mTORC1 is influenced by growth factors, energy status, oxygen levels, and amino acid availability and is involved in a wide variety of processes, including aging, autophagy, stem cell and immune function, cellular senescence, and protein translation. Dysregulation of the mTOR signaling pathway is associated with various pathologies, including cancer, rheumatoid arthritis, epilepsy, neurodegenerative diseases, and diabetes. Cayman's mTOR/mLST8/Raptor Complex (human, recombinant) protein can be used for enzyme activity assays.Synonyms: mTOR:, FKBP12-Rapamycin Complex-associated Protein, FK506-binding Protein 12-Rapamycin Complex-associated Protein 1, Mammalian Target of Rapamycin, Mechanistic Target of Rapamycin, Rapamycin and FKBP12 Target 1, Rapamycin Target Protein 1, Serine/Threonine-protein Kinase mTOR, mLST8:, G protein beta Subunit-like, Gable, GbetaL, Target of Rapamycin Complex Subunit LST8, TORC Subunit LST8, Mammalian Lethal with SEC13 Protein 8, Raptor:, Regulatory-associated Protein of mTOR, RPTOR, p150 Target of Rapamycin-scaffold Protein, p150 TOR-scaffold Protein. Purity: >58% estimated by SDS-PAGE. Source: Active recombinant human N-terminal DYKDDDDK-tagged mTOR, N-terminal His-tagged mLST8, and N-terminal His-tagged Raptor expressed in insect cells. Amino Acids: 1,362-2,549 (mTOR), 2-326 (mLST8), and 2-1,335 (Raptor). MW: 137 (mTOR), 37 (mLST8), and 150 (Raptor) kDa. Formulation: (Request formulation change), 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 80 ng/µl DYKDDDDK peptide, and 20% glycerol. Host: Insect cells. UniProt Accession #: P42345 (mTOR), Q9BVC4 (mLST8), Q8N122 (Raptor).