MRE11 (human, recombinant, aa 1-411)

Meiotic recombination 11 homolog (MRE11) is an endo- and exonuclease and member of the MRE11-RAD50-NBS1 (MRN) complex, which is involved in DNA double-strand break repair. It exists as a homodimer and is composed of an N-terminal nuclease domain with five phosphodiesterase motifs, two dimerization motifs, and a Nijmegen breakage syndrome protein 1 (NBS1) interaction domain, a capping domain, and a C-terminal domain that contains two DNA-binding domains on either side of the RAD50 interaction domain. Alternative splicing of MRE11 produces two main isoforms, MRE11 isoform 1, which is the full-length isoform, and MRE11 isoform 2, which lacks exon 16. MRE11 is expressed in proliferative tissues such as testis and spleen and localizes to the nucleus following DNA damage. It is involved in homologous recombination (HR) and non-homologous end joining (NHEJ) and forms complexes with two dsDNA ends (synaptic complex) or a single ssDNA or dsDNA end (branched complex). Knockdown of MRE11 sensitizes cells to radiation in vitro, an effect that can be rescued by expression of MRE11 isoform 1 or 2. Overexpression of MRE11 increases tumor volume in a breast cancer mouse xenograft model. Mutations in MRE11 are associated with ataxia-telangiectasia-like disorder (ATLD) and cancer. Cayman's MRE11 (human, recombinant, aa 1-411) protein has a calculated molecular weight of 74.7 kDa.Synonyms: Double-strand Break Repair MRE11, Meiotic Recombination 11. Purity: >90% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Recombinant human N-terminal His- and GST-tagged MRE11 expressed in E. coli. Amino Acids: 1-411. MW: 74.7 kDa. Formulation: (Request formulation change), 50 mM Tris-HCl, pH 7.5, 200 mM sodium chloride, 20% glycerol. UniProt Accession No: P49959.