MMP-17, Recombinant, Human, aa39-531 (Matrix Metalloproteinase 17, MT4-MMP)

Matrix metalloproteinase 17 (MMP­17) is a zinc metalloproteinase that is an integral membrane protein (1). It belongs to the membrane­type MMP (MT­MMP) subfamily, and is also known as MT4­MMP. It is associated with the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (1). It has the potential to function as a pro­tumor necrosis factor­a (TNF­a) convertase (2). Unlike MMP­14 (MT1­MMP), it is a poor activator of pro­MMP­2. MMP­17 is relatively poor at digesting components of the extracellular matrix, but does cleave fibrinogen and fibrin (2). MMP­17 is known to activate the aggrecanase ADAMTS­4 through proteolysis (3). MMP­17 is expressed in many tissues with the highest levels seen in the brain, colon, ovary, testis, and kidney papilla (4). It has been detected in several human cancers, including gliomas, prostate carcinomas, and breast carcinomas (5). Recombinant Human MMP­17 was expressed without its GPI anchoring sequence, resulting in its secretion as a soluble protein. Source: Recombinant protein corresponding to Ala39-Ser531 (Arg125Pro) & (Ala182Thr), from human MMP-17, fused to 6xHis-Tag at C-terminal, NS0-derived. Biological Activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. Specific Activity: >150 pmol/min/ug, as measured under the described condition. Molecular Weight: ~56-65kD, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.