MLX-interacting protein (MLXIP), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-217aa. Protein Length: Full Length of Isoform 5. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MDEQGCEHTS RTEDPFIQPT DFGPSEPPLS VPPPISPVLP LVPPPATALN PPAPPTFHQP QKFAGVNKAP SVITHTASAT LTHDAPATTF SQSQGLVITT HHPAPSAAPC GLALSPVTRP PQPRLTFVHP KPVSLTGGRP KQPHKIVPAP KPEPVSLVLK NARIAPGEPG GETQCGAPPD PEGCFPIPKA FKLVTTTTTL VCTCMRTHIH LNETKVS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds DNA as a heterodimer with MLX and activates transcription. Binds to the canonical E box sequence 5'-CACGTG-3'. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. Reference: A novel heterodimerization domain, CRM1, and 14-3-3 control subcellular localization of the MondoA-Mlx heterocomplex.Eilers A.L., Sundwall E., Lin M., Sullivan A.A., Ayer D.E.Mol. Cell. Biol. 22:8514-8526(2002). Function: Binds DNA as a heterodimer with MLX and activates transcription. Binds to the canonical E box sequence 5'-CACGTG-3'. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.