Mitogen-activated protein kinase kinase kinase 14 (MAP3K14), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 400-655aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ATHQLRLGRG SFGEVHRMED KQTGFQCAVK KVRLEVFRAE ELMACAGLTS PRIVPLYGAV REGPWVNIFM ELLEGGSLGQ LVKEQGCLPE DRALYYLGQA LEGLEYLHSR RILHGDVKAD NVLLSSDGSH AALCDFGHAV CLQPDGLGKS LLTGDYIPGT ETHMAPEVVL GRSCDAKVDV WSSCCMMLHM LNGCHPWTQF FRGPLCLKIA SEPPPVREIP PSCAPLTAQA IQEGLRKEPI HRVSAAELGG KVNRAL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. Reference: "Regulation of the NF-kappaB-inducing kinase by tumor necrosis factor receptor-associated factor 3-induced degradation." Liao G., Zhang M., Harhaj E.W., Sun S.C. J. Biol. Chem. 279:26243-26250(2004). Function: nan