Mitochondrial-processing peptidase subunit beta (PMPCB), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 44-489aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: STQAATQVVL NVPETRVTCL ESGLRVASED SGLSTCTVGL WIDAGSRYEN EKNNGTAHFL EHMAFKGTKK RSQLDLELEI ENMGAHLNAY TSREQTVYYA KAFSKDLPRA VEILADIIQN STLGEAEIER ERGVILREMQ EVETNLQEVV FDYLHATAYQ NTALGRTILG PTENIKSISR KDLVDYITTH YKGPRIVLAA AGGVSHDELL DLAKFHFGDS LCTHKGEIPA LPPCKFTGSE IRVRDDKMPL AHLAIAVEAV GWAHPDTICL MVANTLIGNW DRSFGGGMNL SSKLAQLTCH GNLCHSFQSF NTSYTDTGLW GLYMVCESST VADMLHVVQK EWMRLCTSVT ESEVARARNL LKTNMLLQLD GSTPICEDIG RQMLCYNRRI PIPELEARID AVNAETIREV CTKYIYNRSP AIAAVGPIKQ LPDFKQIRSN MCWLRD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins (Probable). Preferentially, cleaves after an arginine at position P2. Required for PINK1 turnover by coupling PINK1 mitochondrial import and cleavage, which results in subsequent PINK1 proteolysis. Reference: "Mitochondrial processing peptidase regulates PINK1 processing, import and Parkin recruitment." Greene A.W., Grenier K., Aguileta M.A., Muise S., Farazifard R., Haque M.E., McBride H.M., Park D.S., Fon E.A. EMBO Rep. 13:378-385(2012). Function: nan