Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-209aa. Protein Length: Full Length. Tag Info: N-terminal GST-tagged. Target Protein Sequence: MEGGGGSGNK TTGGLAGFFG AGGAGYSHAD LAGVPLTGMN PLSPYLNVDP RYLVQDTDEF ILPTGANKTR GRFELAFFTI GGCCMTGAAF GAMNGLRLGL KETQNMAWSK PRNVQILNMV TRQGALWANT LGSLALLYSA FGVIIEKTRG AEDDLNTVAA GTMTGMLYKC TGGLRGIARG GLTGLTLTSL YALYNNWEHM KGSLLQQSL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Reference: "Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death." Guo Y., Cheong N., Zhang Z., De Rose R., Deng Y., Farber S.A., Fernandes-Alnemri T., Alnemri E.S. J. Biol. Chem. 279:24813-24825(2004). Function: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.