Mitochondrial glutamate carrier 2 (Slc25a18), mouse, recombinant

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 1-320aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MIACRMSSQD LSISAKLING GIAGLVGVTC VFPIDLAKTR LQNQQGKDVY RGMTDCLMKT ARAEGFLGMY RGAAVNLTLV TPEKAIKLAA NDFLRQLLMQ DGTQRNLKME MLAGCGAGIC QVVITCPMEM LKIQLQDAGR LAVCHQASAS ATPTSRPYST GSTSTHRRPS ATLIARELLR TQGLSGLYRG LGATLLRDIP FSIIYFPLFA NLNQLGVSEL TGKASFTHSF VAGCTAGSVA AVAVTPLDVL KTRIQTLKKG LGEDTYSGVT DCARKLWTQE GPAAFMKGAG CRALVIAPLF GIAQGVYFIG IGERILKCFE-. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Responsible for the transport of glutamate from the cytosol into the mitochondrial matrix with the concomitant import of a proton (symport system). Reference: "Trans-synaptic interaction of GluRdelta2 and Neurexin through Cbln1 mediates synapse formation in the cerebellum." Uemura T., Lee S.J., Yasumura M., Takeuchi T., Yoshida T., Ra M., Taguchi R., Sakimura K., Mishina M. Cell 141:1068-1079(2010). Function: nan