Microtubule-associated protein tau (Mapt) (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 2-733aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: ADPRQEFDTM EDHAGDYTLL QDQEGDMDHG LKESPPQPPA DDGAEEPGSE TSDAKSTPTA EDVTAPLVDE RAPDKQAAAQ PHTEIPEGIT AEEAGIGDTP NQEDQAAGHV TQGRREGQAP DLGTSDWTRQ QVSSMSGAPL LPQGLREATC QPSGTRPEDI EKSHPASELL RRGPPQKEGW GQDRLGSEEE VDEDLTVDES SQDSPPSQAS LTPGRAAPQA GSGSVCGETA SVPGLPTEGS VPLPADFFSK VSAETQASQP EGPGTGPMEE GHEAAPEFTF HVEIKASTPK EQDLEGATVV GVPGEEQKAQ TQGPSVGKGT KEASLQEPPG KQPAAGLPGR PVSRVPQLKA RVASKDRTGN DEKKAKTSTP SCAKAPSHRP CLSPTRPTLG SSDPLIKPSS PAVSPEPATS PKHVSSVTPR NGSPGTKQMK LKGADGKTGA KIATPRGAAS PAQKGTSNAT RIPAKTTPSP KTPPGSGEPP KSGERSGYSS PGSPGTPGSR SRTPSLPTPP TREPKKVAVV RTPPKSPSAS KSRLQTAPVP MPDLKNVRSK IGSTENLKHQ PGGGKVQIIN KKLDLSNVQS KCGSKDNIKH VPGGGSVQIV YKPVDLSKVT SKCGSLGNIH HKPGGGQVEV KSEKLDFKDR VQSKIGSLDN ITHVPGGGNK KIETHKLTFR ENAKAKTDHG AEIVYKSPVV SGDTSPRHLS NVSSTGSIDM VDSPQLATLA DEVSASLAKQ GL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Mapt at 2 µg/mL can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU),the EC50 is 436.1-518.6 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Reference: n/a. Function: nan