Microbial collagenase (colA), partial, Clostridium perfringens, recombinant

Organism: Clostridium perfringens (strain 13 / Type A). Source: E.coli. Expression Region: 90-370aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: NKIYTFDELN RMNYSDLVEL IKTISYENVP DLFNFNDGSY TFFSNRDRVQ AIIYGLEDSG RTYTADDDKG IPTLVEFLRA GYYLGFYNKQ LSYLNTPQLK NECLPAMKAI QYNSNFRLGT KAQDGVVEAL GRLIGNASAD PEVINNCIYV LSDFKDNIDK YGSNYSKGNA VFNLMKGIDY YTNSVIYNTK GYDAKNTEFY NRIDPYMERL ESLCTIGDKL NNDNAWLVNN ALYYTGRMGK FREDPSISQR ALERAMKEYP YLSYQYIEAA NDLDLNFGGK N. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known, saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities, the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen (By similarity). Reference: "Gene duplication and multiplicity of collagenases in Clostridium histolyticum." Matsushita O., Jung C.-M., Katayama S., Minami J., Takahashi Y., Okabe A. J. Bacteriol. 181:923-933(1999). Function: nan