mFluor(TM) Violet 550-streptavidin conjugate

Absorbance (nm): 527. Correction Factor (260 nm): 0.474. Correction Factor (280 nm): 0.306. Extinction coefficient (cm^-1 M^-1): 90000 (PBS). Excitation (nm): 527. Emission (nm): 550. Quantum yield: 0.31 (PBS). mFluor(TM) Violet 550-streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor(TM) Violet 550 fluorescent dyes. Its high affinity for biotin conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor(TM) Violet 550 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by a violet laser, mFluor (TM) Violet 550 exhibits a bright and photostable emission of green fluorescence at 550 nm. mFluor(TM) Violet 550 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.