mFluor(TM) Violet 545-streptavidin conjugate

Absorbance (nm): 398. Correction Factor (260 nm) 1.08. Correction Factor (280 nm) 0.496. Extinction coefficient (cm^-1 M^-1): 20000 (PBS). Excitation (nm): 393. Emission (nm): 543. Quantum yield. 0.15 (PBS). The mFluor(TM) Violet 545-streptavidin conjugate is a tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor(TM) Violet 545 fluorescent dyes. It has a strong binding affinity for biotin conjugates, making it useful in various biochemical applications for detecting specific proteins, protein motifs, nucleic acids, and other molecules. This concept is essential for workflows such as western blots, flow cytometry, imaging and microscopy, and microplate assays, as well as for purification workflows aimed at targeted fractionation. The conjugation of mFluor(TM) Violet 545 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. When excited by a violet laser, mFluor(TM) Violet 545 emits bright and photostable green fluorescence at 543 nm. mFluor(TM) Violet 545 streptavidin conjugates are particularly useful as second-step reagents for indirect immunofluorescent staining when used with biotinylated primary antibodies.