Methyl-CpG-binding domain protein 2 (MBD2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 145-411aa. Protein Length: Partial. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: ESGKRMDCPA LPPGWKKEEV IRKSGLSAGK SDVYYFSPSG KKFRSKPQLA RYLGNTVDLS SFDFRTGKMM PSKLQKNKQR LRNDPLNQNK GKPDLNTTLP IRQTASIFKQ PVTKVTNHPS NKVKSDPQRM NEQPRQLFWE KRLQGLSASD VTEQIIKTME LPKGLQGVGP GSNDETLLSA VASALHTSSA PITGQVSAAV EKNPAVWLNT SQPLCKAFIV TDEDIRKQEE RVQQVRKKLE EALMADILSR AADTEEMDIE MDSGDEA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009). Function: nan