Metalloendopeptidase OMA1, mitochondrial (OMA1) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 14-524aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: HVFFRFNSLS NWRKCNTLAS TSRGCHQVQV NHIVNKYQGL GVNQCDRWSF LPGNFHFYST FNNKRTGGLS STKSKEIWRI TSKCTVWNDA FSRQLLIKEV TAVPSLSVLH PLSPASIRAI RNFHTSPRFQ AAPVPLLLMI LKPVQKLFAI IVGRGIRKWW QALPPNKKEV VKENIRKNKW KLFLGLSSFG LLFVVFYFTH LEVSPITGRS KLLLLGKEQF RLLSELEYEA WMEEFKNDML TEKDARYLAV KEVLCHLIEC NKDVPGISQI NWVIHVVDSP IINAFVLPNG QMFVFTGFLN SVTDIHQLSF LLGHEIAHAV LGHAAEKAGM VHLLDFLGMI FLTMIWAICP RDSLALLCQW IQSKLQEYMF NRPYSRKLEA EADKIGLLLA AKACADIRAS SVFWQQMEFV DSLHGQPKMP EWLSTHPSHG NRVEYLDRLI PQALKIREMC NCPPLSNPDP RLLFKLSTKH FLEESEKEDL NITKKQKMDT LPIQKQEQIP LTYIVEKRTG S. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized OMA1 at 5 µg/ml can bind human BZW2, the EC50 of human BZM2 is 45.42-72.22 µg/ml. Form: Lyophilized powder. Buffer: Lyophilized from 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Metalloprotease that is part of the quality control system in the inner membrane of mitochondria. Following stress conditions that induce loss of mitochondrial membrane potential, mediates cleavage of OPA1 at S1 position, leading to OPA1 inactivation and negative regulation of mitochondrial fusion. May also cleave UQCC3 under these conditions. Its role in mitochondrial quality control is essential for regulating lipid metabolism as well as to maintain body temperature and energy expenditure under cold-stress conditions. Reference: n/a. Function: nan