Membrane protein (M), partial, human coronavirus 229E, recombinant

Organism: Human coronavirus 229E (HCoV-229E). Source: E.coli. Expression Region: 96-225aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: ANSFRLFRRA RTFWAWNPEV NAITVTTVLG QTYYQPIQQA PTGITVTLLS GVLYVDGHRL ASGVQVHNLP EYMTVAVPST TIIYSRVGRS VNSQNSTGWV FYVRVKHGDF SAVSSPMSNM TENERLLHFF. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins. Reference: "Sequence analysis of the membrane protein gene of human coronavirus 229E." Jouvenne P., Richardson C.D., Schreiber S.S., Lai M.M.C., Talbot P.J. Virology 174:608-612(1990). Function: nan