Melanopsin (Opn4), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 1-474aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MNSPSESRVP SSLTQDPSFT ASPALLQGIW NSTQNISVRV QLLSVSPTTP GLQAAAWVPF PTVDVPDHAH YTLGTVILLV GLTGMLGNLT VIYTFCRNRG LRTPANMLII NLAVSDFLMS FTQAPVFFAS SLYKKWLFGE TGCKFYAFCG AVFGIVSMIT LTAIAMDRYL VITRPLATIG MRSKRRTALV LLGVWLYALA WSLPPFFGWS AYVPEGLLTS CSWDYVTFTP LVRAYTMLLF CFVFFLPLLI IIFCYIFIFR AIRETGRACE GCGESPLRRR QWQRLQSEWK MAKVALIVIL LFVLSWAPYS TVALVGFAGY SHILTPYMSS VPAVIAKASA IHNPIIYAIT HPKYRAAIAQ HLPCLGVLLG VSGQRSHPSL SYRSTHRSTL SSQSSDLSWI SGQKRQESLG SESEVGWTDT ETTAAWGAAQ QASGQSFCSH DLEDGEVKAP SSPQEQKSKT PKTKRHLPSL DRRM. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Photoreceptor that binds cis-retinaldehydes . Contributes to pupillar reflex, photoentrainment and other non-image forming responses to light . May be involved in the optokinetic visual tracking response . May be involved in the regulation of retinal hyaloid vessel growth and regression . Reference: "Phosphorylation of rat melanopsin at Ser-381 and Ser-398 by light/dark and its importance for intrinsically photosensitive ganglion cells (ipRGCs) cellular Ca2+ signaling." Fahrenkrug J., Falktoft B., Georg B., Hannibal J., Kristiansen S.B., Klausen T.K. J Biol Chem 289:35482-35493(2014). Function: nan