Melanoma-associated antigen 12 (MAGEA12), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-314aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MPLEQRSQHC KPEEGLEAQG EALGLVGAQA PATEEQETAS SSSTLVEVTL REVPAAESPS PPHSPQGAST LPTTINYTLW SQSDEGSSNE EQEGPSTFPD LETSFQVALS RKMAELVHFL LLKYRAREPF TKAEMLGSVI RNFQDFFPVI FSKASEYLQL VFGIEVVEVV RIGHLYILVT CLGLSYDGLL GDNQIVPKTG LLIIVLAIIA KEGDCAPEEK IWEELSVLEA SDGREDSVFA HPRKLLTQDL VQENYLEYRQ VPGSDPACYE FLWGPRALVE TSYVKVLHHL LKISGGPHIS YPPLHEWAFR EGEE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Not known, though may play a role tumor transformation or progression. In vitro promotes cell viability in melanoma cell lines. Reference: "MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines." Yang B., O'Herrin S.M., Wu J., Reagan-Shaw S., Ma Y., Bhat K.M., Gravekamp C., Setaluri V., Peters N., Hoffmann F.M., Peng H., Ivanov A.V., Simpson A.J., Longley B.J. Cancer Res. 67:9954-9962(2007). Function: nan