Melanoma-associated antigen 10 (MAGEA10), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-369aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MPRAPKRQRC MPEEDLQSQS ETQGLEGAQA PLAVEEDASS STSTSSSFPS SFPSSSSSSS SSCYPLIPST PEEVSADDET PNPPQSAQIA CSSPSVVASL PLDQSDEGSS SQKEESPSTL QVLPDSESLP RSEIDEKVTD LVQFLLFKYQ MKEPITKAEI LESVIRNYED HFPLLFSEAS ECMLLVFGID VKEVDPTGHS FVLVTSLGLT YDGMLSDVQS MPKTGILILI LSIVFIEGYC TPEEVIWEAL NMMGLYDGME HLIYGEPRKL LTQDWVQENY LEYRQVPGSD PARYEFLWGP RAHAEIRKMS LLKFLAKVNG SDPRSFPLWY EEALKDEEER AQDRIATTDD TTAMASASSS ATGSFSYPE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression. Reference: "MAGE-A10 is a nuclear protein frequently expressed in high percentages of tumor cells in lung, skin and urothelial malignancies." Schultz-Thater E., Piscuoglio S., Iezzi G., Le Magnen C., Zajac P., Carafa V., Terracciano L., Tornillo L., Spagnoli G.C. Int. J. Cancer 129:1137-1148(2011). Function: Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.